文章摘要

潘越.西达苯胺联合传统化疗药物促进急性T淋巴细胞系的凋亡[J].内科急危重症杂志,2021,27(2):148-152

西达苯胺联合传统化疗药物促进急性T淋巴细胞系的凋亡

Chidamide combined with traditional chemotherapy promotes apoptosis of T lymphoblastic leukemia cell lines

DOI：10.11768/nkjwzzzz20210216

英文关键词:

基金项目:国家自然科学基金（No:81873456）

作者	单位	E-mail
潘越	华中科技大学同济医学院附属同济医院	zhuxuehaitj@163.com

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中文摘要:

目的：探讨西达苯胺联合伊达比星或左旋门冬酰胺酶对急性T淋巴细胞白血病细胞系Jurkat、MOLT4、CCRF-CEM增殖及凋亡的影响。方法：CCK8法检测西达苯胺、伊达比星、左旋门冬酰胺酶单药或两药联合对Jurkat、MOLT4、CCRF-CEM细胞的增殖抑制作用，流式细胞术检测细胞凋亡，Westernblot法检测凋亡相关蛋白的表达。结果：西达苯胺、伊达比星、左旋门冬酰胺酶单药作用于细胞，细胞的增殖抑制呈现药物浓度及作用时间依赖性。流式细胞术检测发现，西达苯胺联合伊达比星或左旋门冬酰胺酶作用于细胞相较于任何一种单药诱导细胞凋亡更加明显（均 P <0.05），具有明显的协同效应（ CI <0.7），且联合用药组细胞凋亡蛋白表达水平较单药组显著升高。结论：西达苯胺联合伊达比星或左旋门冬酰胺酶具有协同抑制急性T淋巴细胞白血病细胞系Jurkat、MOLT4、CCRF-CEM增殖，促进细胞凋亡的作用。

英文摘要:

Objective: To investigate the effect of chidamide combined with idarubicin (IDA) or L-Asparaginase (L-ASP) on the proliferation and apoptosis of acute T lymphoblastic leukemia cell line Jurkat, MOLT4 and CCRF-CEM. Methods: CCK8 kit was used to determine the anti-proliferation effect of different concentrations of chidamide, IDA and L-ASP. The cell apoptosis was analyzed by flow cytometry and the expression of apoptosis-associated protein was detected by Western boltting. Results: Chidamide, IDA and L-ASP displayed inhibitory effects on proliferation of cell lines in a dose- and time-dependent manner. The combination of chidamide with IDA or L-ASP led to higher apoptosis than chidamide, IDA or L-ASP alone in the same concentration gradient after 48 h ( P <0.05), and the cooperation indexes of the two drugs were all less than 0.7. In addition, the expression of apoptosis-associated protein was significantly higher in combination group than in the single group. Conclusion: Combination of chidamide with IDA or L-ASP shows a significantly synergistic effects on proliferation inhibition and apoptosis of acute T-cell lymphoblastic leukemia cell line Jurkat, MOLT4 and CCRF-CEM, which may be associated with apoptosis-associated protein expression.

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